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Joywell Foods rebrands as Oobli, unveils first food product featuring oubli fruit sweet protein (brazzein)

by agrifood
December 15, 2022
in FoodTech
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Oobli – which was originally planning to debut with beverages​​ – told us it had switched to confectionery as chocolate bars “were a better fit for a Q4/holiday launch, so it is more of a market/seasonality adjustment to the sequence.”​

The chocolate bars - available for pre-sale on Oobli’s website​​ - contain unsweetened chocolate, chicory root fiber, organic coconut sugar, cocoa butter, ground vanilla beans, and oubli fruit sweet protein (a.k.a. brazzein).

Each 32g serving of the Oobli 70% cacao bar contains 150 calories, 3g sugar, and 9g fiber. As a point of contrast, Lindt 70% dark chocolate (sweetened with sugar) contains 190 cals, 9g sugar, and 2g fiber per 30g serving; while Lily’s 70% extra dark no sugar added chocolate (sweetened with erythritol and stevia extract) contains 130 calories, 0g sugar, and 8g fiber.

2,000 times sweeter than sugar

The ultra-sweet protein brazzein – which is up to 2,000 times sweeter than sugar – is found in tiny quantities in oubli fruit, which makes it prohibitively expensive to extract.

Instead, Oobli is producing the same protein in fermentation tanks via a genetically engineered strain of yeast. However, as no detectible modified DNA from the host microbe (a pichia yeast strain) is in the final product, it will not trigger a ‘bioengineered’ label.

As the protein is chemically identical to the protein found in the fruit, the company is listing it as ‘oubli fruit sweet protein​’ on the ingredients list, said the firm: “Oobli is a sweet protein platform technology and consumer goods company. The oubli fruit sweet protein is one of the proteins on the platform.” ​

Oobli - which raised $25m earlier this year​ - ​has not confirmed the regulatory status of its sweet protein although FoodNavigator-USA understands it is going through the GRAS process.

Sweet protein potential​

While stevia and monk fruit sweeteners have improved significantly as firms have homed in on the more sugar-like steviol glycosides such as Reb M, sweet proteins add an exciting new dimension to the sugar-reduction toolbox, claimed CEO Ali Wing in an interview with this publication in May.

While some stakeholders such as Germany’s Nomad Bioscience​​ (which expresses thaumatin II in transgenic plants instead and expects to be on the market by 2024) have argued in regulatory filings​​​ ​that producing sweet proteins via microbial fermentation is not scalable or cost competitive, Wing said she begged to differ.

“I wouldn’t have joined the company to be a commercial leader if I didn’t think we had a commercially viable strategy around costs of production, and we do believe we have a very clear path to cost competitiveness with sugar.”​​​

Sugary taste profile without trade-offs?​

But what’s exciting about sweet proteins in the first place?

They are all different, said Wing, with some a few hundred times sweeter than sugar (brazzein) and others (thaumatin) a couple of thousand times sweeter, but in general they’re exciting because they don’t come with the trade-offs that you get with most commercially-available high intensity sweeteners.

“Taste is the number one attraction, but a protein-based solution ​​​[for sweetness] is also attractive as it doesn’t trigger blood sugar ​​ or interact negatively with the gut microbiome; our bodies are very good at digesting and managing protein.”​​​

Consumer-friendly labeling?​

Another plus point is that while Oobli’s proteins are not extracted from fruits, they are bioidentical to the proteins found in fruits, so can be labeled in a very consumer-friendly way (eg. ‘oubli fruit sweet protein’​), said Wing.

“We’re excited about using multiple sweet proteins together, as there are differences between them in things like solubility and heat stability and taste profiles, so thaumatin has a really nice upfront sweetness, for example, while brazzein is really soluble. The one that’s quite different is miraculin, which is a taste modifier ​​​[rather than something that itself imparts sweetness], and it’s a different beast, if you will.” ​​

Further reading:

· Joywell Foods raises $25m as it gears up to launch beverages featuring sweet proteins​​

· Sweet proteins… finally ready for prime time?​​

· Amai Proteins finalizing initial closing of $100m round as it gears up for launch of ‘designer’ sweet protein in 2023​​

Sweet proteins include:​ ​​

  • Thaumatin I and II​:​​ from katemfe fruit, found in West Africa, 2,000-3,000x sweeter than sucrose, readily soluble in cold water
  • Brazzein​​​: from oubli fruit, found in West Africa, 500-2,000x sweeter than sucrose, heat-stable, stable in a wide range of pH conditions, and highly soluble
  • Pentadin​​​: also from oubli fruit, 500x sweeter than sucrose
  • Curculin​​​: from fruit of Curculingo latifolia​​​ found in West Africa, exhibits sweet-tasting and taste-modifying properties
  • Mabinlin​​​: from seeds of Chinese plant Mabinlang (Capparis masaikai Levl.)
  • Monellin​​​: from serendipity berries, found in West Africa
  • Lysosome​​​: from egg whites
  • Miraculin​​​: from miracle fruit, found in West Africa. It is not sweet in and of itself, but binds to sweet receptors to cause sour-tasting acidic foods such as citrus fruits to be perceived as sweet

The chocolate bars - available for pre-sale on Oobli’s website - contain unsweetened chocolate, chicory root fiber, organic coconut sugar, cocoa butter, ground vanilla beans, and oubli fruit sweet protein (a.k.a. brazzein). Image credit: Oobli



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